2 Percent Chlorhexidine Solution Can Be Used to Decontaminate Soiled Bone Segments

Objective: To scientifically evaluate a method that can reliably be used to cleanse a contaminated bone segment. Methods: 54 evenly cut porcine tibial bone segments were divided into 3 groups of 18. All segments were dropped into a broth containing genetically altered Staphylococcus aureus that emitted photons. The 3 groups were then exposed to either normal saline or 2% versus 4% solutions of chlorhexidine. The contaminated bone segments were then imaged before and after exposure to the solutions at intervals of 5, 10, 20, 30, and 60 minutes. For each time point, photon counts were compared to that which was measured for the respective bone segment at time zero. Results: A rapid decrease in bacterial numbers was noticed after 5 minutes in both chlorhexidine solutions. This difference was noted to be significant as compared to the normal saline solution group. Both chlorhexidine solutions were equally effective at the specified time points and required as little as 20 minutes to remo more...